The ETT in vitro alternative assays provide evidence based toxicity data relevant to the new European Union Regulation REACH (Registration, Evaluation, Authorisation and restriction of CHemicals) for assessing toxicity and safety for the protection of human health and the environment.
The ETT alternative toxicity screening service is promoting 3Rs.
The paradigm exploits a hybrid system of cells and engineered dishes through which the functional endpoint (electrophysiology) of active cells (e.g. neurons) can be assessed.
ETT service also provides fundamental information for assessing efficacy of neuro-targeted new drugs during pre-clinical development and test.
ETT assesses single cell electrophysiology and ion channel functions in response to compound interaction by patch clamp technique. Patch Clamp is a highly accurate and sensitive evaluation method and provides complementary information to MEA based electrophysiology.
Cellular Toxicity is evaluated by studying uncontrolled increase in cytoplasmic calcium. Quantitative image analysis on fluorescent Ca2+ indicator Fura 2-AM is used to asses dose-response curves.
ETT provides its clients a complete report for the best chemical profiling. Toxicity and safety screening reports include information about cell viability and cytotoxicity assessed by: