MEA Mid-throughput electrophysiological recording

ETT provides you with an innovative system to detect neurotoxicity

The ETT in vitro alternative assays provide evidence based toxicity data relevant to the new European Union Regulation REACH (Registration, Evaluation, Authorisation and restriction of CHemicals) for assessing toxicity and safety for the protection of human health and the environment.
The ETT alternative toxicity screening service is promoting 3Rs.

The paradigm exploits a hybrid system of cells and engineered dishes through which the functional endpoint (electrophysiology) of active cells (e.g. neurons) can be assessed.
ETT service also provides fundamental information for assessing efficacy of neuro-targeted new drugs during pre-clinical development and test.

Single-cell electrophysiology – Patch Clamp

ETT assesses single cell electrophysiology and ion channel functions in response to compound interaction by patch clamp technique. Patch Clamp is a highly accurate and sensitive evaluation method and provides complementary information to MEA based electrophysiology.

Calcium signaling and cytotoxicity

Cellular Toxicity is evaluated by studying uncontrolled increase in cytoplasmic calcium. Quantitative image analysis on fluorescent Ca2+ indicator Fura 2-AM is used to asses dose-response curves.

Cell viability and cytotoxicity

ETT provides its clients a complete report for the best chemical profiling. Toxicity and safety screening reports include information about cell viability and cytotoxicity assessed by:

  • lactate dehydrogenase (LDH) assay
  • Mithochondrial activity assay
  • Customized cell staining

More services

In partnership with the Laboratory of Pathophysiological Analyses and Research (LARF) of University of Genoa, we are pleased to offer you a full set of EU ECVAM validated in vitro assay for:

  • cytotoxicity
  • phototoxicity
  • cellular proliferation
  • anti/pro- oxidant potential
  • anti/pro-inflammatory activity
  • sensitizing potential
  • biotransformation enzymatic activity of phase 1 and phase 2
  • gene and protein expression and localization of markers of the cellular damage, oxidative stress, irritation/inflammation, cellular activation.

For more details, please visit http://www.larf-dimes.onweb.it/en/home

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